This site is intended for healthcare professionals

Sign in
Go to /sign-in page

You can view 5 more pages before signing in

DNA sequencing

Authoring team

The sequence of nucleotides of isolated fragments of DNA can be deduced by at least two different groups of chemical reactions. The most famous of these methods are those of Sanger - the dideoxy technique - and Maxam & Gilbert - the cleavage technique. The latter technique is now outmoded.

The production of machines that can undertake these reactions repeatedly and at speed has added impetus to the Human Genome Project.

The Human Genome Project originally aimed to map the nucleotides contained in a human haploid reference genome (more than three billion)

  • the "genome" of any given individual is unique; mapping "the human genome" involves sequencing multiple variations of each gene
  • project did not study the entire DNA found in human cells; some heterochromatic areas (about 8% of the total genome) remain unsequenced
  • project got underway in 1990, and was declared complete in 2003

Reference:

Related pages

Create an account to add page annotations

Create a free account

Add information to this page that would be handy to have on hand during a consultation, such as a web address or phone number. This information will always be displayed when you visit this page

The content herein is provided for informational purposes and does not replace the need to apply professional clinical judgement when diagnosing or treating any medical condition. A licensed medical practitioner should be consulted for diagnosis and treatment of any and all medical conditions.

Connect

Copyright 2024 Oxbridge Solutions Limited, a subsidiary of OmniaMed Communications Limited. All rights reserved. Any distribution or duplication of the information contained herein is strictly prohibited. Oxbridge Solutions receives funding from advertising but maintains editorial independence.